The method of Serial Femtosecond nanocrystallography (SFX) provides a novel concept for structure determination, where X-ray diffraction “snapshots” are collected from a fully hydrated stream of nanocrystals, using femtosecond pulses at the high energy X-ray free-electron laser, the Linac Coherent Light Source [1]. Diffraction data of nanocrystals of large membrane protein complexes are collected at room temperature in liquid jet. As femtosecond pulses are briefer than the time-scale of most damage processes, femtosecond nanocrystallography overcomes the problem of X-ray damage in crystallography. Data collected at 8keV showed that the concept of fs crystallography extends to atomic resolution and can resolve new structural elements [2]. The talk will also include exciting results of SFX on G-protein coupled receptors [3], which can be directly delivered to the X-ray beam in a lipidic cubic phase jet. The talk will also show the future prospective of time resolved femtosecond nanocrystallography [4] towards molecular movies of light-driven reactions in photosynthesis.