Engineering, production and structural characterisation of a novel synthetic PPR protein — ASN Events
  1. Schedule
  2. Session 7: Poster Session B - Including Happy Hour & Trade Display
  3. Engineering, production and structural characterisation of a novel synthetic PPR protein

Engineering, production and structural characterisation of a novel synthetic PPR protein (#231)

Kate Shearston 1 , Peter Kindgren 1 , Benjamin S Gully 1 , Kunal S Shah 1 , Ian D Small 1 , Charlie S Bond 1
  1. University of Western Australia, Crawley, WA, Australia

Pentatricopeptide repeat (PPR) proteins consist of tandem arrays of degenerate 35 amino acid repeats. PPR proteins participate in multiple steps of organellar RNA metabolism including RNA editing, stabilisation, translation, degradation and splicing. In plants, PPR protein mutations result in cytoplasmic male sterility, seed development defects and other impairments.

Recent studies characterising the mode of PPR protein RNA interaction have provided an opportunity for the rational design of synthetic PPR proteins engineered to bind a specific RNA sequence.

Here we report the design, production and structural characterisation of a novel synthetic PPR protein generated from a consensus sequence.